利用ND1、ITS1序列研究三种背角无齿蚌（Anodontawoodiana）分类学问题     

赵颖  陈修报  杨健  刘洪波 《海洋与湖沼》2011,42(4):619-624

利用ND1、ITS1序列差异对采自太湖、在形态上被认为是背角无齿蚌、圆背角无齿蚌、椭圆背角无齿蚌的三种个体进行鉴定,并与从NCBI中下载到的同属不同种褶纹冠蚌的ND1、ITS1序列进行对比,分析了基于Kimura2-parameter模型参数得到的遗传距离,并构建了NJ树。结果表明,本研究中所有背角无齿蚌种间遗传距离变...  相似文献   

Numerical studies of methane production from Class 1 gas hydrate accumulations enhanced with carbon dioxide injection   总被引：2，自引：0，他引：2  

M.D. White  S.K. WurstnerB.P. McGrail 《Marine and Petroleum Geology》2011,28(2):546-560

Class 1 gas hydrate accumulations are characterized by a permeable hydrate-bearing interval overlying a permeable interval with mobile gas, sandwiched between two impermeable intervals. Depressurization-induced dissociation is currently the favored technology for producing gas from Class 1 gas hydrate accumulations. The depressurization production technology requires heat transfer from the surrounding environment to sustain dissociation as the temperature drops toward the hydrate equilibrium point and leaves the reservoir void of gas hydrate. Production of gas hydrate accumulations by exchanging carbon dioxide with methane in the clathrate structure has been demonstrated in laboratory experiments and proposed as a field-scale technology. The carbon dioxide exchange technology has the potential for yielding higher production rates and mechanically stabilizing the reservoir by maintaining hydrate saturations. We used numerical simulation to investigate the advantages and disadvantages of using carbon dioxide injection to enhance the production of methane from Class 1 gas hydrate accumulations. Numerical simulations in this study were primarily concerned with the mechanisms and approaches of carbon dioxide injection to investigate whether methane production could be enhanced through this approach. To avoid excessive simulation execution times, a five-spot well pattern with a 500-m well spacing was approximated using a two-dimensional domain having well boundaries on the vertical sides and impermeable boundaries on the horizontal sides. Impermeable over- and under burden were included to account for heat transfer into the production interval. Simulation results indicate that low injection pressures can be used to reduce secondary hydrate formation and that direct contact of injected carbon dioxide with the methane hydrate present in the formation is limited due to bypass through the higher permeability gas zone.  相似文献   

An installation system of deepwater risers by an S-lay vessel     

段梦兰  王懿  何宁  李丽娜  陈邦敏 《中国海洋工程》2011,25(1):139-148

Along with the consumption increase of the petroleum products,more countries have transferred their attentions to the offshore fields,especially the deepwater oil and gas reserves.For deepwater exploitation,the risers must be installed to act as the conduits connecting surface platforms to subsea facilities.In this paper,the typical risers sorted by different classes are introduced,and the correspondent installation methods are presented.By investigating the recent projects performed in the deepwater hot spots,and combining the challenges of HYSY201 for riser installation,a lifting device developed for assisting riser installation is proposed and detailed to satisfy the installation of deepwater risers in the LW3-1 Gas Field of in the South China Sea.Tests on both the functions and performances of such a new system exhibit the satisfaction of meeting all challenging requirements of HYSY201 for application to riser installation in waters up to a depth of in the South China Sea.  相似文献   

基于Quickbird影像的数字线划图数据更新          下载免费PDF全文

范志坚  付蓉 《海洋测绘》2011,31(2):72-75

伴随着我国国民经济建设的快速发展,有必要对现势性较差的1:1万数字线划图(DLG)数据进行更新.常用数据更新方法比较繁琐,成本比较高,制作的周期也较长.因此,对基于Quickbird影像对1:1万DLG数据更新的相关技术进行了探讨.  相似文献   

饲料中硒和α-硫辛酸对皱纹盘鲍稚鲍抗氧化反应的影响     

陈齐勇  张文兵  麦康森  马洪明  刘付志国 《中国海洋大学学报(自然科学版)》2011,41(5):38-42

本研究利用2×3双因素试验设计,分别添加0和1 mg/kg的硒,0、800和3 200 mg/kg的α-硫辛酸,配制成6种半精制饲料,在流水系统中养殖初始体质量为(0.43±0.00)g的皱纹盘鲍稚鲍(Haliotis discus hannaiIno)16周,探讨硒和硫辛酸对皱纹盘鲍稚鲍抗氧化反应的影响。结果表明:硒对皱纹盘鲍肝胰脏中谷胱甘肽过氧化物酶(GPX)和总抗氧化力(T-AOC)有显著影响,当饲料中不添加硫辛酸时,硒显著提高了肝胰脏GPX活性及T-AOC值。饲料中添加800 mg/kg硫辛酸时,皱纹盘鲍肝胰脏中的GPX活性、超氧化物歧化酶(SOD)活性和T-AOC值均显著增加,谷胱甘肽(GSH)的含量也有所增加,但差异不显著。然而,添加3 200 mg/kg硫辛酸时,GPX活性和T-AOC值显著下降。尽管没有统计学上的显著差异,饲料中硒和硫辛酸对皱纹盘鲍稚鲍抗氧化反应的影响表现出了潜在的交互作用。总的来说,饲料中同时添加1mg/kg硒和800 mg/kg硫辛酸时,皱纹盘鲍稚鲍能够表现出较好的抗氧化状态。  相似文献   

壳聚糖酶基因在解脂耶氏酵母中的表达及重组酶性质研究   总被引：1，自引：0，他引：1  

刘万顺  王娇  杨艳  韩宝芹  董文  刘光磊  池振明 《中国海洋大学学报(自然科学版)》2011,41(12):58-62

为构建高效表达壳聚糖酶工程菌株,以Microbacteriumsp.基因组为模板扩增壳聚糖酶基因,目的基因与表达载体pINA1317连接构建重组质粒,重组质粒NotⅠ酶切线性化后转化解脂耶氏酵母Y.liPolytica Po1h。所得结果为PCR扩增得到约1000bp的特异性片段,序列分析表明含有壳聚糖酶全长基因,开放阅读框801bp,编码266个氨基酸残基。转化子酶活力为10.4U/mL,是原菌株酶活力的3.15倍。重组蛋白经DEAE-Sepharose FF分离纯化,达到电泳纯,SDS-PAGE显示单一条带,分子量约41kDa。重组酶反应最适温度为45℃,最适pH为5.6,Km和Vmax分别是0.926mg/mL和6.15U/mL。质谱分析酶解产物主要为三糖和五糖。实现了壳聚糖酶在解脂耶氏酵母中的分泌表达,为壳聚糖酶的工业化生产奠定了理论基础。  相似文献   

国家西部测图工程产品模式设计与实践——以1:50000影像地形图为例     

苏山舞  于荣花  殷红梅  李维庆  陈代蓉 《地球信息科学学报》2011,13(6):763-769

为实现国家西部1∶50 000地形图空白区测图工程:丰富产品种类,拓展服务领域的设计宗旨,根据ISO 9000对产品的概念,提出了西部测图工程整体产品模式,并对其进行细化,建立了国家两部测图工程地形图产品模式.本文以西部测图工程1∶50 000影像地形图的设计与实现为实例,详细介绍了1∶50 000影像地形图测绘新产品...  相似文献   

基于HJ-1B/IRS的重庆市热岛效应监测应用     

罗小波  陈丹  刘明皓  刘强 《地球信息科学学报》2011,13(6):833-839

本文利用MODIS数据反演大气透射率,利用HJ-1B/CCD进行分类,并反演地表比辐射率.在此基础上,借鉴单窗算法,利用HJ-1B/IRS数据反演得到地表温度,并利用MODIS温度产品对反演结果进行了初步验证.最后利用热场变异指数进一步分析重庆的热岛空间分布特征,并对NDVI与NDBI对热岛效应的影响进行了分析.其结论...  相似文献   

导航电子地图增量式更新服务模式     

郭鹏  崔铁军 《地理信息世界》2011,(4):57-61

为了解决导航电子地图数据生产成本高昂、更新周期长等问题,提出了由导航变化数据发现、分析、采集、制作、发布构成的导航电子地图增量式更新闭环服务模式,将公众和导航数据用户等非专业数据生产人员纳入到导航电子地图数据维护和更新框架,可以提高数据的现势性,而且大大地降低生产和应用成本,为导航电子地图服务提供了一种可操作性强的参考...  相似文献   

Numerical study on the bifurcation of the North Equatorial Current     

Yulong Liu  Qi Wang  Jun Song  Xiande Zhu  Xiaoqing Gong  Fang Wu 《中国海洋大学学报(英文版)》2011,10(4):305-313

A 1.5-layer reduced-gravity model forced by wind stress is used to study the bifurcations of the North Equatorial Current(NEC).The authors found that after removing the Ekman drift,the modelled circulations can serve well as a proxy of the SODA circulations on the σθ=25.0 kg m～-3 potential density surface based on available long-term reanalysis wind stress data.The modelled results show that the location of the western boundary bifurcation of the NEC depends on both zonal averaged and local zero wind stress curl latitude.The effects of the anomalous wind stress curl added in different areas are also investigated and it is found that they can change the strength of the Mindanao Eddy(ME),and then influence the interior pathway.  相似文献